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The peptide is engineered with a fatty-acid moiety to extend its circulation time (half-life ~6 days), allowing for once-weekly dosing [2]

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For silencing experiments, fully differentiated SGBS cells were transfected with 100 nM of siCtrl or sip53 (Thermo Fisher Scientific, Waltham, MA, United States) by using Lipofectamine RNAiMax (Thermo Fisher Scientific, Waltham, MA, United States) following the providers instructions