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The presence of an increase in protein levels at the expected molecular weight on an SDS-PAGE gel after induction with IPTG is a good indication of successful protein expression

L-Glutathione (GSH): The Free Thiol Group Is Both the Source of Activity and Instability In the L-glutathione molecule, the thiol group of cysteine is in a completely free state

Chem Sci

Cell Uptake and Metabolism of Glutathione To date, two mechanisms of glutathione uptake into mammalian cells are known [19]

This included the CIII subunit core protein 2 and CIV subunit MTCO1, indicating that GSH-deficiency altered protein expression independently of the overall activity of CIII and CIV