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There is general agreement that M3 receptors are primarily responsible for the normal contraction of the urinary bladder , although M2 receptors are also present, and may act to oppose sympathetically mediated smooth muscle relaxation mediated by beta-3 receptors (Hedge, 2009

They were then permeabilized in 0.2% Triton X-100 for 10 min and blocked with 8% goat serum for 1 h at room temperature

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In some embodiments, the otic formulation or composition comprises about 0.09% by weight of the poloxamer