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Glutamate cotransmission in cholinergic, GABAergic and monoamine systems: contrasts and commonalities

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The TGR sample (1/50 volume cell culture) was applied to a 2.5 ml column, which was then washed with 50 ml of TE after which TGR was eluted in ten 1 ml fractions of 1 mM NADPH in TE

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