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Relative mRNA expression was quantified using the 2 Ct method

Commonly affected areas include the parametrium, anterior rectal wall, colon, ureters, bladder and posterior vaginal fornix

Furthermore, descending inhibitory pathways, which modulate the visceral pain signal by releasing neurotransmitters such as opioids, noradrenaline, dopamine and 5-HT [94], are down-regulated in IBS patients as indicated by an increased somatic sensitivity [95]