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The dose depends on the current health status of the animal, its age, and weight

Subcutaneous adipose tissue in the buttocks is not vascular enough to deliver enough methyl-B12 fast enough to produce a significant clinical response in such a short period of time

It acts as a cofactor for methionine synthase and methylmalonyl-CoA mutase, enzymes that are critical for one-carbon metabolism as well as the catabolism of odd-chain fatty acids and branched-chain amino acids (Rucker et al., 2007)