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Site-directed mutagenesis Site-directed mutagenesis of the GST clone was performed using a QuikChange mutagenesis kit (Stratagene) according to the manufacturers protocol

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Large scale research projects can get multiple vials from the same manufacturing batch to eliminate batch to batch variations

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10.4103/1463-1741.90327 33 KawadaH.BlessingK.KiyotaT.WoolmanT.WinchesterL.KadorP