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After washing with TBST, membranes were incubated for 1 h at room temperature with the secondary antibodies conjugated to horseradish peroxidase (HRP) anti-rabbit IgG (Cell Signaling) or anti-mouse IgG (Cell Signaling)

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Local reactions may include erythema, pruritus, induration, or mild pain at the injection site, potentially affecting five to ten percent of patients

Clinical studies, conflicting guidelines, and fragmented records leave most patients paralyzed