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Briefly, the pre-weighed striatal tissues were homogenized in a 0.1 N perchloric acid solution, centrifuged at 13 000 g and 4 C for 30 min, and eluted using a mobile phase consisting of 17% acetonitrile, 100 L/L triethylamine, 75 mM NaH 2 PO 4 , 1.7 mM 1-octane sulfonic acid, and 25 M EDTA