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Transient transfections were performed using 25 kDa linear polyethylenimine (PEI) (purchased from Polysciences Inc

Breakdown of nucleic acids to uric acid leads to a significant increase in uric acid excretion, which can result in acute kidney injury (Mughal 2010), while hyperkalemia (high levels of potassium), hyperphosphatemia (high levels of phosphorus), and secondary hypocalcemia (low levels of calcium) can quickly progress to medical emergencies (Wilson 2012)

In general such protocol consists of three major steps: cell lysis and solubilisation of the GST-fusion protein (see cartoon below), recovery of the fusion from the lysate and elution of the GST fusion

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Davenport 2010