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Primary mouse peritoneal macrophages, mouse macrophage-like RAW 264.7 cells, and human monocytic THP-1 cells were transfected with GSTP encoding expression vector (pcDNA3.1-Flag-GSTP) or empty vector (pcDNA3.1), followed by stimulation of LPS (500 ng/ml) for 18 h, and the levels of HMGB1 in the culture medium were determined by ELISA

All procedures complied with German animal welfare regulations and received prior approval from the ethics committees and local authorities in Dresden, including the ethics committee of the Technische Universitt Dresden and the Landesdirektion Sachsen (registration number: TVV07/2021)

2 for the metabolic pathways affected by SULT)

However, if youre already taking Vitamin C, Vitamin E, or another glutathione booster shot or antioxidant supplement separately, be mindful of not exceeding safe daily limits