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Indeed, this magnitude appeared to increase with the Brnsted nuc values of the corresponding reactions of LMW thiolates with each electrophile, which in turn correlate with the amount of charge transferred in the transition state ( In the case of protein cysteines, the effect of persulfidation on the nucleophilic reactivity compared with the corresponding thiol is variable, and depends not only on the particular protein but also on the electrophile as well as on experimental conditions such as pH

Aconitase activity To evaluate a functional consequence of oxidative stress, the activity of the redox-sensitive enzyme, aconitase, was measured using the Aconitase Assay Kit (Cayman Chemical, Ann Arbor, MI, USA) following the manufacturer's instructions

She told the nurse you going to actually make me wait till after breakfast before you will help me and the nurse replied Oh youlll be fine, iLl come back later