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After incubation, cells were washed twice with PBS and solubilized in 1.0 ml detergent solution (0.1 M NaOH and 0.1% SDS) by slowly scrapping the monolayer with pipette tip and flushing the detergent solution several times

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Laboratories investigating vascular biology, wound healing models, and endothelial cell behaviour have incorporated this peptide into their research designs to examine how growth factor signalling influences vessel formation at the cellular level

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