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Subsequently, the Colorimetric probe reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm

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IR-induced mitochondrial stress triggers the release of mtDNA into the cytoplasm and extracellular space, where it functions as a damage-associated molecular pattern (DAMP) that activates pattern recognition receptors such as Toll-like receptors (TLRs) and NOD-like receptors (NLRs)