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10 L of each serial dilution is then added to 90 L of blank plasma for final concentrations of 0.01, 0.02, 0.05, 0.1, 0.2, 1, 10, 20, 50, and 100 g/mL

doi:10.1038/srep22386

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49, 50, 51 Abnormalities in zinc and copper in ASD patients Interestingly, a large proportion of the patients in the series demonstrated mild abnormalities in zinc and/or copper concentrations