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This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]

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The form, dose, and timing are worth recording, and further workup becomes necessary if the elevation persists once those sources are accounted for, or if symptoms, examination findings, or other labs raise concern

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