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Overnight (22:0010:00 h) fasting blood glucose (FBG) was measured with an Accu-Chek reflectance meter with Accu-Chek active test strips (Roche Diabetes Care, Mannheim, Germany) on experimental days P7, P14, P21, P28, and P38

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The epidermis also features tight junction proteins that enhance barrier integrity and includes four primary cell types: keratinocytes (forming the water barrier), melanocytes (pigment production), Langerhans cells (immune defense), and Merkel cells (touch receptors) [226, 227]