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2.2 Primary culture of myocytes and thermal stress trial 2.2.1 Primary culture of myocytes Juvenile fish, each weighing approximately 5 g, were dissected under sterile conditions to obtain muscle tissue, which was then cut into pieces and transferred to a 24-well cell culture plate

Pivaloylcarnitine - the conjugate formed when pivaloyl groups are eliminated using carnitine

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These biological and environmental discrepancies limit external validity and severely constrain the generalizability of preclinical findings to real-world clinical settings

Buxton GV, Wood ND, Dyster S (1988) Ionisation constants of .OH and HO2