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NTS prepared as previously described and cultured in M199 supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum were incubated at 37 C in a clear bottom flat well plate in a 5% CO 2 incubator overnight

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subjects had a tan complexion, with fairly dry to fairly oily skin

Together, these results support that DMF activates the TSS pathway and thus contributes to the regulation of cellular oxidative and ferroptosis stress response