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Western blotting Sample preparation Pulverized liver tissue (20 mg) was homogenized in ice cold 1x RIPA buffer (50 mM Tris, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate and 0.1% sodium dodecyl sulfate (SDS), pH 8) using a glass dounce homogenizer

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KPV, a naturally occurring tripeptide derived from -MSH, is being researched for its potent anti-inflammatory properties and ability to downregulate pro-inflammatory cytokines

doi: 10.1371/journal.pone.0072365 71 XuJ-LXiaRSunZ-HSunLMinXLiuCet al