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However, prolonged or intense physical activity can deplete intracellular GSH levels because GSH is oxidized to its disulfide form, glutathione disulfide (GSSG), during ROS detoxification [69,70]
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Properties of hydrogels: biocompatibility, tunability, and degradability Hydrogels are highly hydrated, three-dimensional polymeric networks that combine structural robustness with remarkable versatility, making them ideal candidates for precision biomedical applications [126]
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For GSSG competition assay (Extended Data Figure 4l), the 2x reaction buffer consisted of 10LM [ 3 H]GSSG, 5mM GSSG, 150mM KCl, and 50mM HEPES (pH 7.4)
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