Biosens Bioelectron 72:247254 He B et al (2014) Effects of Pyrroloquinoline quinine on oxidative stress-induced apoptosis of Schwann cells and its mechanism
Inconsistent signals across plates are most commonly caused by temperature differences between the plate and reagent at the time of addition (ensure both are equilibrated to room temperature for 30 minutes before mixing), insufficient cell lysis (increase mixing time or speed), or signal read outside the stable window (read plates within 10 minutes to 1 hour after stabilization)
Co-immunoprecipitation (co-IP) After treatment, the cells were lysed in an ice-cold co-IP buffer containing 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM EDTA, and 0.5% NP-40, supplemented with a protease inhibitor cocktail (Roche, Cat
Magnification of up to a thousand times allows detection of pathological changes within the intestinal epithelium [124]
doi:10.1007/s40261-015-0368-5 HerxheimerA, Petrie KJ