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The HRP enzyme substrate is then incubated in the wells

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The plates were incubated at 30 degrees for 3-6 days and photographs were taken

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Aging is defined here as an organism-level, progressive functional and systemic process characterized by gradual changes in physiological resilience and homeostatic regulation across the lifespan ( Throughout this review, these constructs are addressed according to the level of evidence discussed: organismal aging in relation to circadian rhythm alterations and sleep architecture