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For analysis, each urine sample was thawed at room temperatures and centrifuged at 3000 g for 10 min, followed by filtration of supernatant through a 0.22 m membrane Syringe Filter PTFE (AZ Chrom s.r.o., Bratislava, Slovak Republic)

To systematically elucidate these heterogeneous factors, the following sections will explore four interconnected pathogenic axes that illustrate how ferroptosis cooperatively disrupts the structural integrity and homeostatic function of periodontal tissues

et al: The interstitial cystitis symptom index and problem index