The membranes were incubated in 1:1000 rabbit anti-GLYR1 antiserum in the blocking buffer overnight at 4 C, washed multiple times with TBST, incubated in 1:10,000 goat anti-rabbit IgG Horseradish Peroxidase (HRP) secondary antibody (PhytoAB Inc.) in TBST for 2 h, washed again with TBST, and then developed with the SuperSignal West Pico PLUS Chemiluminescent Substrate kit (Thermo Scientific)
doi: 10.1016/j.arr.2022.101744 24 ZhangYWWuYLiuXFChenXSuJC
Mostofsky & Simmonds, 2008), through a number of thalamocortical circuits
L-glutathione is a naturally occurring compound found throughout the body that plays a central role in antioxidant activity and cellular health
In view of the pivotal role played by GSH in redox system and the relationship between oxidative stress and autophagy 68 , it is conceivable that GSH depletion-induced oxidative stress and lipid peroxidation may be a key point linking ferroptosis and autophagy in RPE cells
