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10.1038/ismej.2015.151 203

Glutathiones role in liver metabolism and hangover symptom relief: Dysregulation of protein S-glutathionylation and antioxidant enzymes

(2022) 35:e15549

RNA isolation and qPCR RNA was collected from 1.25 10 5 macrophages using the RNeasy Mini Kit (#74104 Qiagen) as per the manufacturers protocol

The three-dimensional structure files of core target proteins were retrieved and downloaded in PDB format via the RCSB PDB database ( AutoDock 4.2.6 was also used to evaluate the binding affinity of the protein to the components and to select the lowest energy conformation in the docking simulation