For tobacco-related samples however, with a large nicotine peak, 2MN eluted on the tail of nicotine, which will always create a problem for low-resolution-MS-based confirmation of the presence of a low level of 2MN because with m / z = 84 amu as the MS base peak for both nicotine and 2MN, obtaining a mass spectrum for a small candidate 2MN peak using subtraction of the background signal from a large nicotine tail will not result in a properly-sized m / z = 84 amu signal for proper MS pattern concordance
dilution due to spike volume was accounted for in calculations
Edgar, D., Dawson, A., Hankey, G., Phillips, M
Nicotine Pouches Market Size (US$ Bn and Million Cans), By Consumer Group, 2020 - 2035 17.10.1
One case still stands out