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Flow cytometry Cells were collected, washed twice with PBS, and stained using 5 l of 7-AAD (BD Biosciences, #559925) and 5 l of annexin V-FITC (BD Biosciences, #556420) in 100 l of annexin V binding buffer (BD Biosciences, #556454) per sample

Several groups require higher vitamin B12 doses due to increased needs, impaired absorption, or specific health conditions

However, paradoxically, excessive Fe accumulation can trigger intracellular iron overload, promoting ferroptosis

Transformed cells were selected by cultivating them on YM medium supplemented with 50 mg/L G418

NMN taken by mouth improves NAD+ production in tissues of all types