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The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the primary antibody (HA601199) at 1/200 dilution for 1 hour at room temperature

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Evidence from clinical trials suggests that specific regimensparticularly time-restricted eating and ADFlead to significant decreases in fasting glucose, glycated hemoglobin (HbA1c), and insulin resistance [13]