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The skin was closed with two 5-0 nylon sutures and the wound was covered with an antibiotic ointment

The following formula was used: Monoamines level determination According to Ciarlone 59 , weighted samples of cortical tissue were added to cold acidified butanol (3 mL) in a small beaker and homogenized for 30 s before being placed in plastic tubes and centrifuged at 5000 rpm for 5 min

Meiosis in triploid all-female fish ( Poeciliopsis , Poeciliidae)