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We demonstrated that the GSH synthesis inhibitor buthionine sulfoximine (BSO) synergistically enhanced L-PAM activity (inducing 24 logs of cell kill) against nine MM cell lines (also in the presence of marrow stroma or cytokines) and in seven primary MM samples (combination indices 100 days, and significantly increased the median event-free survival relative to L-PAM alone

For further confirmation, the results of immunofluorescence, Western blotting, and PCR showed that the nuclear translocation of -catenin was detected in H

However, its use has been supplanted by better-tolerated agents because of concerns regarding numerous adverse side effects (listed below)

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