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Cells were washed in staining buffer (5 mM CaCl 2 , 5 mM glucose, 32 mM HEPES pH7.4, 5 mM KCl, 1 mM MgSO4, 125 mM NaCl), resuspended in 0.3 M of intracellular calcium dye x-Rhod-1 AM (Invitrogen) and incubated in the dark at 37C for 30 min

Functionalizing inorganic nanomaterials with polymers, surfactants, or small organic molecules preserves their unique physicochemical attributes while improving biocompatibility and stability (Kankala et al

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