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S100A9 derived from chemoembolization-induced hypoxia governs mitochondrial function in hepatocellular carcinoma progression
Scientific Activity In biochemical and cell models, 5-AMINO-1MQ inhibits NNMT, reducing methyl transfer from S-adenosylmethionine to nicotinamide and altering downstream methyl-donor and nicotinamide metabolite pools
Higher doses mean more gastrointestinal symptoms
Confocal microscopy imaging Lipid droplet visualization After treatment, cells were washed once with PBS and fixed with 3.7% formaldehyde (Sigma) for 10 min, followed by incubation with 10 M BODIPY 493/503 (MedChemExpress, HY-W090090) diluted in PBS for 30 min at 25 C