Climate Registered Accelerator Spotlight: The Johns Hopkins University Prepared by Elsie Andreyev for The Climate Registry
After washing three times with PBS, cells were fixed with 1 mL 4% paraformaldehyde for 15 min, permeabilized with 1 mL 0.1% Triton X-100 for 10 min at room temperature, and blocked with 1 mL 5% BSA for 30 min
Genetic differences in antioxidant genes encoding MnSOD and GPX1 may alter the detoxification effect of ROS and increase disease risk (18)
1990s2010s Animal research expands into gastrointestinal, wound, vascular and musculoskeletal models
In senescence-accelerated murine models, TERT activation by Epithalon correlates with increased median and maximal lifespan, reduced incidence of cytogenetic anomalies in bone marrow metaphases, and improved resistance to oxidative and genotoxic stress 2,4