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Cytofluorimetric Analysis ECs were detached from culture flasks with 5 mM EDTA at 37C and a total number of 5 10 5 were fixed with FIX & PERM cell permeabilization kit (Societ Italiana Chimici) and incubated in permeabilization solution in ice for 30 with mAb (clone 9) mouse anti-human vimentin (Sigma-Aldrich), mAb (cloneF8/86) mouse anti-human vWF, or mAb (cloneV9) mouse anti-human CK8/18

The experiment was a total loss before it even began

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