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Bile pigment stains green with Halls method

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Yeast cells harvested from cultures in the exponential growth phase were washed with sterile distilled water and resuspended to a final concentration of 10 cells/mL in 100 mM phosphate buffer (pH 7.0) supplemented with 0.1% glucose and 1 mM sodium EDTA

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Drinking enough water throughout the day promotes a safer and more effective experience