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Undertaking both phases in a laboratory/aquarium setting enables the control of many environmental conditions and thus enables a researcher to reduce such variables, but comes at a potential cost: it can lead to situations that are no longer representative of the field and, as such, to potentially unrepresentative results ( Controlling culture conditions prior to a chemosensory assay represents a major challenge, as the list of factors influencing an animals physiology and, with this, its ability to respond naturally to a cue are so complex and diverse ( Figure 1 )