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As it mentioned above, ROS accumulation may cause ER stress ( Selenium is an essential trace element and the function of selenium is mediated in part by its incorporation into slenoproteins, and most of these selenoproteins have antioxidant properties such as glutathione peroxidase (GPX) and selenoprotein P (SEPP1) ( in vivo experiments, the direct regulation of HMSeBA on ER stress signal to porcine intestinal epithelial cells (IPEC-J2) also needs further study

Spermatozoa have the highest selenium content in mammalian tissues, and the need for selenium increases at the onset of spermatogenesis ( 2.5 GPX5 Glutathione peroxidase 5 (GPX5), an epididymis-specifically expressed selenium-free antioxidant enzyme, was first isolated and identified in the epididymal tissues of mice ( During epididymis transport, spermatozoa are vulnerable to intracellular and extracellular ROS attacks and rely on antioxidant scavengers synthesized by the epididymis for protection

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