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the Norleual and D-Nle-Tyr-Ile-NH(CH 2 ) 5 CONH 2 samples were precipitated by adding 3 vol of ice-cold acetonitrile (ACN) and the samples were vortexed vigorously

Research on GLP-1 peptide stability shows that recoveries were unaffected by freezing cycles or storage on ice for up to three hours, but were significantly impaired when samples stood at room temperature for more than one hour in plasma studies

The HuCCT1 human bile duct carcinoma cell line was obtained from the Japanese Cancer Research Resources Bank

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