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Then, 200 L of single-cell suspension was gently added along the wall of the upper chamber containing 0.5% FBS (to avoid the interference from cell proliferation in the process of cell invasion)

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Cys 2 ), cystine-free (Cys 2 -free), cystine-free/GSH-supplemented (750 M) or cystine-free/CysGly-supplemented (750 M) medium, and relative cell numbers were determined