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A list of primers and plasmids are provided as separate Supplementary Data 1

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Given that there was no difference in total GSH between Preparation Condition 1 and Preparation Condition 2, and that free GSH is significantly higher in Preparation Condition 1 than in Preparation Condition 2, these results suggest that EDTA-treatment more effectively prevents spontaneous oxidation of free GSH in plasma than does heparinization of samples

This 28-day window is why vial size and reconstitution volume need to be planned together