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Before use, these peptides must be dissolved in an appropriate diluent to create a solution with a specific concentration

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The Ni-NTA resin was packed into a glass column and washed with 25 column volumes of 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, followed by elution using the same buffer supplemented with 250 mM imidazole and 3 mM CaCl 2

However, it should always be used under the supervision of a healthcare practitioner as an incorrect dosage can lead to adverse side effects such as nausea, dizziness, headache, and fatigue

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