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Equal amounts of total protein (30 g per sample) were resolved by 1012% SDS-PAGE and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore or Invitrogen, Shanghai, China)

It works by supporting synaptic communication, modulating serotonin and dopamine pathways, and boosting antioxidant defenses in the brain

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The phylogenetic tree was constructed by ClustalX version 1.81 and MEGA version 5, with a p -distance distribution, pairwise deletion, and bootstrap analysis of 10,000 repeats

Glossary Damage-associated molecular patterns (DAMPs)