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The selected amino acid site in the nanobody was replaced with a UAG stop codon by site-directed mutagenesis for incorporating ncAA with corresponding primers (Table S5)

HUVEC and HDMEC were maintained in Endothelial Cell Basal Medium (PromoCell) supplemented with 2% FCS, 0.4% endothelial cell growth supplement, 0.1 ng ml 1 recombinant human EGF (rh EGF), 1 ng ml 1 recombinant human basic fibroblast growth factor, 90 g ml 1 heparin and 1 g ml 1 hydrocortisone

doi: 10.1002/1529-0131(199912)42:12 3.0.CO;2-H

A common error is adding 1 mL to a 5 mg vial, yielding 5 mg/mL, then drawing the same 12.5 units and administering 625 mcg when 250 mcg was intended

USA 107 , 1519915204 (2010)