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For liquid chromatographytandem mass spectrometry (LC-MS/MS) analysis, an Easy-nLC 1200 (Thermo Fisher Scientific) was connected inline to an Orbitrap Fusion LUMOS (Thermo Fisher Scientific) equipped with a nanoelectrospray ion source (Thermo Fisher Scientific)

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Klopot A, Baida G, Bhalla P, Haegeman G, Budunova I

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The liver responds to GH by producing IGF-1 , and IGF-1 is what actually drives the downstream tissue effects clinicians associate with growth hormone protein synthesis, satellite-cell activation for muscle repair, lipolysis, collagen synthesis, and bone remodeling