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Separation was performed by gradient chromatography at a flow rate of 0.25 ml/min, starting at 98% B for 3 min, ramping to 5% B over 20 min, hold at 5% B for 1 min, then back to 98% B

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A novel S100 target conformation is revealed by the solution structure of the Ca2+-S100B-TRTK-12 complex

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