Samples were then digested with trypsin (11 L of 0.5 g/L per sample, resuspended in trypsin resuspension buffer containing 20 mM CaCl 2
The amount of 03 mg/d in addition to the requirements of non-pregnant women has been estimated to be sufficient for the normal growth of maternal tissues and the fetus (69,72,73,79)
The definitive way to confirm a peptide is laboratory analysis: high-performance liquid chromatography (HPLC) measures purity, mass spectrometry (MS) confirms molecular identity, and a Limulus Amebocyte Lysate (LAL) assay screens for bacterial endotoxins
van Geldermalsen M, Wang Q, Nagarajah R, Marshall AD, Thoeng A, Gao D, Ritchie W, Feng Y, Bailey CG, Deng N, Harvey K, Beith JM, Selinger CI, O'Toole SA, Rasko JE, Holst J
[3] Chang CH, Tsai WC, Hsu YH, Pang JH