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and hydrophilic factor ( Hy ), which reflects the ability of a molecule to interact with water through polar interactions such as hydrogen bonds or dipole interactions, calculated using alvaDesc software (Alvascience, Lecco, Italy)

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After 48 h of transfection, some cells were seeded into 96-well plates for experiments, and the rest cells were collected for immunoblotting to detect knockdown efficiency

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The study also found that glutathione levels began to return to baseline approximately one month after supplementation ceased, indicating that consistent use is key to maintaining optimal levels