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These were incubated at 37 C and 5% CO 2

ELISA Tests The levels of anti-PR3-hn-hr and anti-MPO in the serum samples collected from the examined women were measured using enzyme-linked immunosorbent assay (ELISA) kits obtained from Euroimmun Medizinische Labordiagnostica AG (Lbeck, Germany)

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Impact of oxidative stress on male and female germ cells: implications for fertility