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(8)Fmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (8) Preparation of Fmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (7)Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin(20DMF)5203DMF3DMF2Fmoc-Pro-OH0.8gDIEA0.4mlTBTU0.56gHOBT0.2gDMF10ml 1.53DMF3DMF2Fmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin In the Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin of step (7), add decapping reagent (DMF solution of 20% piperidine), react at room temperature for 5 minutes, pump Dry, re-add the capping agent and react at room temperature for 20 minutes, wash with isopropanol for 3 times, DMF for 3 times, re-distilled DMF for 2 times, drain, add Fmoc-Pro-OH0.8g, DIEA0.4ml, TBTU0 .56g, HOBT0.2g, DMF10ml, reacted at room temperature for 1.5 hours, dried with a vacuum pump, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with re-distilled DMF, and dried to obtain Fmoc-Pro-Ala- Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin

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Lyophilisation removes moisture from the material and helps support stability before research handling

02 Work sterile Prepare a clean surface and use proper gloves when handling needles, syringes, or vials